Synthesis and Preclinical Studies of [61Cu]-N-(2-Hydroxyacetophenone)glycinate as a Possible PET Radiopharmaceutical

Cu]-N-(2-hydroxyacetophenone)glycinate ([Cu]NHAG) was prepared using in house-made NHAG ligand and [Cu]CuCl2 produced via the Zn(p,x)Cu (180μA proton irradiation, 22MeV, 3.2h) and purified by a ion chromatography method. [Cu]NHAG radiochemical purity was >98% and >99.9% by RTLC and HPLC methods respectively after purification by SPE. [Cu]NHAG was administered into normal and tumor bearing mice followed by biodistribution studies up to 180 minutes. The best tumor accumulation was observed by animal sacrification after 120 min (tumor/muscle and tumor/blood ratios were 25.6 and 3.4 respectively). [Cu]NHAG is a potential PET radiotracer for tumor imaging.